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Friday, January 13, 2012

Eosinophils and asthma in mice

      Asthma is a disease that most Americans have knowledge of from childhood.  In grade school, there were always one or two children in class who either had to have their inhaler at the ready during recess and gym or were unable to take part in playground antics in fear of becoming short of breath.  As years passed, it became less obvious who was afflicted with asthma; as assignments would pile up, recess went away.  Yet, asthma remained.  It is not just an illness of childhood, it can affect people of any age, ethnicity and occupation.  It can be caused in part by genetic factors or by environmental triggers, especially proximity to smokers or excessively smoky areas.  This chronic disease emerges from the adaptive immune system’s response to “environmental assaults” and leads to constriction of the airway, coughing, tightness in the chest and shortness of breath.  The use of an inhaler with a corticosteroid will open the airway back up and allow the afflicted to breathe easy…at least until their next attack.  In order to better combat asthma, it is necessary to understand the mechanisms that make it so dangerous
      Asthma is a “type I hypersensitivity reaction,” meaning that it is caused by the secretion of a particular type of antibody, IgE, following a Th2 immune response. Th2 responses are one of the major types of immunity, and target extracellular pathogens. The Th2 class of helper T cells secrete cytokines that stimulate B cells to produce IgE, which can activate other types of cells to respond to antigens. When these IgE antibodies are targeted against environmental allergens, and not pathogens, type I hypersensitivity results, and when the response occurs in the bronchioles, it results in asthma. A recent study at the Mayo Clinic Arizona on asthma examined the role of eosinophils, a type of specialized white blood cell, in the regulation of dendritic cells and Th2 pulmonary immune responses with T cells in asthmatic mice.  There have been rumblings that the current description of eosinophils as “end-stage destructive effector cells” is not broad enough and that they in fact are involved with the secondary immune responses which lead to the activation and proliferation of memory T cells.  The DCs present the antigen to the lungs; in studies of asthma in mice, including this one, the mice were given additional DCs to better spread the allergen triggering antigen.  If a mouse has fewer eosinophils, then they generally will have reduced Th2 pulmonary abilities.   This study suggests that in allergen-specific T cell responses, eosinophils and DCs work together and are equally important and lead to a Th2 polarized immune response.  The eosinophils were found to have a profound impact on whether the inflammation of the respiratory tract goes by a Th2 pathway or a Th17 (a different “flavor” of immune response) pathway. 
      The relationship between the location of the eosinophils and their abundance, as compared with the accumulation of T cells during an asthma attack, was studied with three types of mice: eosinophil-null (PHIL mice), eosinophil-sufficient (wild type) and eosinophil-low (IL-5-/- mice).  PHIL mice had absolutely no eosinophils in their system, while the IL-5-/- mice had a partial loss of eosinophils compared to the wild type.  The mice were all treated with injections of an innocuous allergen under conditions that trigger asthma (OVA/Alum) and were subsequently exposed to airborne antigens.  The effects of the airborne antigens on the mice were then examined.  The comparisons between the three types of mice reveal information about whether or not the mice were able to accumulate DCs and eosinophils.  The draining lymph nodes of the PHIL mice did not acquire DCs in the 20 hours after they were exposed to the airborne antigens. In contrast, within 20 hours the nodes of the wild type mice acquired both eosinophils and MHC II activated DCs.  The researchers concluded that eosinophils are necessary for DCs to move to the lymph nodes and to cause T cell activation.  The mice that lacked the proper amount of eosinophils could not have the correct Th2 polarization in their lungs once they were exposed to an antigen, as the T cells were not properly activated in the LDLNs. 
      There is certainly more to be learned about eosinophils, DCs, T cells and their role in causing asthma.  The researchers suggest that eosinophils as monitors of localized immune responses can provide wonderful insights into the wider role of eosinophils in the immune system, as their impact on inflammation must certainly involve more than just allergies and asthma. 

Jacobsen, E. A., Zellner, K. R., Colbert, D., Lee, N. A., & Lee, J. J. (2011). Eosinophils regulate dendritic cells and Th2 pulmonary immune responses following allergen provocation. The Journal of Immunology, 187, 6059-6068.

Post by Jessie Solcz

1 comment:

  1. Its a nice blog informing about asthma medications

    ReplyDelete